|
KMID : 0545120150250122110
|
|
Journal of Microbiology and Biotechnology
2015 Volume.25 No. 12 p.2110 ~ p.2115
|
|
|
A Liquid-Based Colorimetric Assay of Lysine Decarboxylase and Its Application to Enzymatic Assay
|
|
Kim Yong-Hyun
Sathiyanarayanan Ganesan
Kim Hyun-Joong
Bhatia Shashi Kant
Seo Hyung-Min
Kim Jung-Ho
Song Hun-Seok
Kim Yun-Gon
Park Kyung-Moon
Yang Yung-Hun
|
|
|
Abstract
|
|
|
|
A liquid-based colorimetric assay using a pH indicator was introduced for high-throughput monitoring of lysine decarboxylase activity. The assay is based on the color change of bromocresol purple, measured at 595 nm in liquid reaction mixture, due to an increase of pH by the production of cadaverine. Bromocresol purple was selected as the indicator because it has higher sensitivity than bromothymol blue and pheonol red within a broad range and shows good linearity within the applied pH. We applied this for simple determination of lysine decarboxylase reusability using 96-well plates, and optimization of conditions for enzyme overexpression with different concentrations of IPTG on lysine decarboxylase. This assay is expected to be applied for monitoring and quantifying the liquid-based enzyme reaction in biotransformation of decarboxylase in a high-throughput way.
|
|
|
KEYWORD
|
|
|
Cadaverine, Lysine decarboxylase, pH indicator, High-throughput colorimetric assay, Bromocresol purple
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|